DOT

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Legend: RNA signal: green, DNA staining: magenta

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Probe source:	HL04319 (Flybase link CG8538 , FlyFish insitu , BDGP insitu new)
stage	images	Annotation
stage1-3		Ubiquitous (Unlocalized) Annotated by Ronit Expressed Stage 1-3 Medium expression (21-40ms exp) Maternal
stage4-5		Embryonic localization patterns Stage 4-5 Localized Apical localization Apical enrichment Degradation maternal transcripts Apical clusters Degraded partially stage 4 Diffuse apical localization Degraded Expressed Weak expression (>41ms exp) Degraded partially stage 5 Maternal
stage6-7		Expressed Weak expression (>41ms exp) Stage 6-7 Ubiquitous (Unlocalized)
stage8-9		Ubiquitous (Unlocalized) Stage 8-9 Zygotic Expression in API vent blastoderm nuclei Weak expression (>41ms exp) Subset blastoderm nuclei Expressed Blastoderm nuclei
stage10-		Expressed Blastoderm nuclei Stage 10- Zygotic Salivary glands lumen Salivary glands
Probe source:	RE49017 (Flybase link CG8538 , FlyFish insitu , BDGP insitu new)
stage	images	Annotation
stage1-3		Ubiquitous (Unlocalized) Stage 1-3 Expressed Annotated by Ronit Weak expression (>41ms exp) Maternal
stage4-5		Zygotic Stage 4-5 Embryonic localization patterns Random blastoderm nuclei Expressed Zygotic starting stage 5 Discrete apical foci Pole cell exclusion Cytoplasmic foci Exclusionary patterns Apical enrichment Localized Blastoderm nuclei Subset blastoderm nuclei Subcellular localization patterns Weak expression (>41ms exp)
stage6-7		Ubiquitous (Unlocalized) Stage 6-7 Weak expression (>41ms exp) Expressed
stage8-9		Stage 8-9 Zygotic Localized Embryonic localization patterns Expressed Blastoderm nuclei Subcellular localization patterns Subset blastoderm nuclei Weak expression (>41ms exp) Cytoplasmic foci

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