DOT

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Legend: RNA signal: green, DNA staining: magenta

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Probe source:	GH26280 (Flybase link CG32705 , FlyFish insitu , BDGP insitu new)
stage	images	Annotation
stage1-3		Weak expression (>41ms exp) Ubiquitous (Unlocalized) Stage 1-3 Expressed Annotated by Ronit Maternal
stage4-5		Localized Exclusionary patterns Stage 4-5 Expressed Pole cell exclusion Weak expression (>41ms exp) Embryonic localization patterns Maternal
stage6-7		Zygotic starting stage 6 Zygotic Subset yolk nuclei Expression in mesoderm Blastoderm nuclei Ubiquitous (Unlocalized) Random yolk nuclei Weak expression (>41ms exp) Subset blastoderm nuclei Expressed Stage 6-7 Yolk Nuclei Maternal
stage8-9		Accumlation nuclear subregion Subcellular localization patterns Nuclei-associated localization Stage 8-9 Weak expression (>41ms exp) Intranuclear accumulation Subset blastoderm nuclei Random blastoderm nuclei Zygotic Expression in API Embryonic localization patterns Blastoderm nuclei Expressed Expression in mesoderm Expresssion in amnioserosa Localized
stage10-		Subcellular localization patterns Blastoderm nuclei Expressed Stage 10- Zygotic Localized
Probe source:	GM06219 (Flybase link CG32705 , FlyFish insitu , BDGP insitu new)
stage	images	Annotation
stage1-3		Non-expressed Annotated by Christina Stage 1-3
stage4-5		Non-Expressed Stage 4-5
stage6-7		Non-Expressed Stage 6-7
stage8-9		Non-expressed Stage 8-9

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