DOT

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Legend: RNA signal: green, DNA staining: magenta

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Probe source:	RE22432 (Flybase link CG3114 , FlyFish insitu , BDGP insitu new)
stage	images	Annotation
stage1-3		Ubiquitous (Unlocalized) Medium expression (21-40ms exp) Stage 1-3 Expressed Annotated by Ronit Maternal
stage4-5		Apical enrichment Degradation maternal transcripts Subcellular localization patterns Degraded partially stage 4 Degraded Zygotic starting stage 5 Blastoderm nuclei Localized Stage 4-5 Embryonic localization patterns Pole cell localization Discrete apical foci Weak expression (>41ms exp) Random blastoderm nuclei Zygotic Subset blastoderm nuclei Expressed Cytoplasmic foci
stage8-9		Localized Expression in ectoderm Blastoderm nuclei Stage 8-9 Accumlation nuclear subregion Subcellular localization patterns Expression in API Nuclei-associated localization Weak expression (>41ms exp) Zygotic Random blastoderm nuclei Cytoplasmic foci Expressed
stage10-		Salivary glands Subcellular localization patterns Blastoderm nuclei Localized Sensory sys CNS Expressed Zygotic PNS Brain Stage 10- vent nerve cord

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