DOT

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Legend: RNA signal: green, DNA staining: magenta

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Probe source:	GM05229 (Flybase link CG8421 , FlyFish insitu , BDGP insitu new)
stage	images	Annotation
stage1-3		Non-expressed Annotated by Christina Stage 1-3
stage4-5		Weak expression (>41ms exp) Stage 4-5 vent blastoderm nuclei Subset blastoderm nuclei Zygotic Blastoderm nuclei ant blastoderm nuclei Zygotic starting stage 4 Expressed
stage6-7		Expression in mesoderm vent blastoderm nuclei Weak expression (>41ms exp) Subset blastoderm nuclei Zygotic Blastoderm nuclei Stage 6-7 Expressed
stage8-9		Stage 8-9 Non-expressed
Probe source:	AT24863 (Flybase link CG8421 , FlyFish insitu , BDGP insitu new)
stage	images	Annotation
stage1-3		Cytoplasmic foci Annotated by Ronit Expressed Stage 1-3 Ubiquitous (Unlocalized) Weak expression (>41ms exp) Maternal
stage4-5		Zygotic Expressed Zygotic starting stage 4 Apical exclusion Pole cell exclusion Stage 4-5 Blastoderm nuclei Degraded vent localization Cytoplasmic foci Localized Degraded partially stage 5 Embryonic localization patterns Weak expression (>41ms exp) vent blastoderm nuclei Exclusionary patterns Subcellular localization patterns Subset blastoderm nuclei
stage6-7		Zygotic ant blastoderm nuclei Stage 6-7 Blastoderm nuclei vent localization Localized Expressed Expression in API vent blastoderm nuclei Expression in mesoderm Embryonic localization patterns Subset blastoderm nuclei
stage8-9		Zygotic Blastoderm nuclei ant blastoderm nuclei Cytoplasmic foci Localized vent localization Expressed Expression in mesoderm vent blastoderm nuclei Embryonic localization patterns Expression in neuroblasts Subset blastoderm nuclei Stage 8-9 Subcellular localization patterns
stage10-		Expressed Localized Stage 10-

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